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Image Search Results
Journal: Journal of proteome research
Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures
doi: 10.1021/pr100164x
Figure Lengend Snippet: A. Cartoon depicting stages of melanoma progression in skin. Melanocytes reside in the epidermal/dermal junction. Early-stage radial growth phase melanoma cells descend into the papillary dermis and spread laterally. Invasion deep into the dermis characterizes melanomas in the vertical groth phase. B. Cartoon of a skin organ culture (SOC). SOCs are maintained at the air-liquid interface. The inset depicts a hematoxylin & eosin stained section collected on day 12 of culture.
Article Snippet:
Techniques: Organ Culture, Staining
Journal: Journal of proteome research
Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures
doi: 10.1021/pr100164x
Figure Lengend Snippet: A. Quantitative RT-PCR analysis of the expression of tenascin-C (TN-C) mRNA in WM983-A (VGP) and WM1158 (MGP) melanoma cells compared to human melanocytes, propagated in vitro. The data are expressed as the mean±standard deviation from three independent experiments. B. Immunoblot analysis depicting the level of TN-C in the VGP and MGP melanoma cell lines and melanocytes. C. Immunofluorescence analysis of melanocytes and VGP and MGP melanoma cell lines, probed with antibody to TN-C (pseudocolored green) and actin (pseudocolored red). The cells were counterstained with fluorescent DAPI (pseudocolored blue).
Article Snippet:
Techniques: Quantitative RT-PCR, Expressing, In Vitro, Standard Deviation, Western Blot, Immunofluorescence
Journal: Journal of proteome research
Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures
doi: 10.1021/pr100164x
Figure Lengend Snippet: A. Quantitative RT-PCR analysis of α-actinin-4 (ACN4) expression in the WM983-A (VGP) and WM1158 (MGP) melanoma cell lines and in melanocytes. The data are expressed as the mean ±standard deviation from three independent experiments. B. Immunoblot analysis of ACN4 expression in the VGP and MGP melanoma cell lines and in melanocytes. C. Immunofluorescence analysis of melanocytes and VGP and MGP melanoma cell lines, probed with antibody to ACN4 (pseudocolored green) and actin (pseudocolored red). The cells were counterstained with fluorescent DAPI (pseudocolored blue).
Article Snippet:
Techniques: Quantitative RT-PCR, Expressing, Standard Deviation, Western Blot, Immunofluorescence